Trofinetide Impurity 16, CAS 847952-36-7, ist eine definierte niedermolekulare verwandte Substanz mit der Summenformel C8H12N2O2 und einer Molekülmasse von 168,19 g/mol. Die Zusammensetzung weist auf eine polare, stickstoffhaltige Verunreinigung hin, die eher als Abbauprodukt oder Synthesenebenprodukt zu werten ist als als einfacher Kohlenwasserstoffrest. Für die Impuritätscharakterisierung werden solche Spezies üblicherweise mittels RP-LC in Kopplung mit hochauflösender MS erfasst, während LC-MS/MS die Spurenquantifizierung und LC-UV eine orthogonale Bestätigung ermöglicht. Die Strukturzuordnung beruht auf Retentionsverhalten, exakter Masse und Fragmentierung, da amidische, Harnstoff- oder heterocyclische Isomere ähnliche chromatographische Signale zeigen können.
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