La impureza 2-((carboxymethyl)amino)-3-((carboxymethyl)thio)propanoic acid presenta grupos carboximetilo | amino y tio que le confieren carácter polar y capacidad para formar zéwitteriones y quelatos con metales | afectando la estabilidad y pureza del producto final. Su formación suele relacionarse con reacciones secundarias durante etapas de carboximetilación o sulfonación en síntesis orgánica. Para su identificación y cuantificación se recomiendan técnicas analíticas sensibles como HPLC con detección por UV o LC-MS y confirmación mediante RMN. El control estricto de límites | limpieza de procesos y técnicas de purificación como intercambio iónico y cromatografía reversa mitigan su impacto en calidad y rendimiento.
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