Estriol Related Compound A; 9, 11-didehydroestriol; (8S,13S,14S,16R,17R)-13-methyl-7,8,12,13,14,15,16,17-octahydro-6H-cyclopenta[a]phenanthrene-3,16,17-triol
Descripción
La Impureza A de Estriol EP, también denominada Estriol Related Compound A y 9,11-didehidroestriol, es un producto de degradación esteroideo oxigenado asociado con el estriol bajo condiciones de estrés oxidativo y deshidratativo. Esta impureza estrogénica insaturada, CAS 246021-20-5, presenta fórmula molecular C18H22O3 y peso molecular de 286.37 g/mol. La pérdida de saturación en la región C9-C11 modifica el armazón esteroideo conjugado y puede alterar la polaridad cromatográfica y la respuesta UV. Su determinación en trazas suele requerir separación ortogonal por HPLC o UHPLC acoplada a LC-MS/MS y, cuando procede, espectrometría de masas de alta resolución para confirmación. Es relevante para el perfil de impurezas y la evaluación de estabilidad de materiales que contienen estriol.
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