Tri-beta-Alanina HCl, CAS 78089-27-7, es la sal hidrocloruro de un derivado de aminoácido polibásico con fórmula C9H17N3O4·HCl y peso molecular de 231,25 + 36,46 g/mol. Estructuralmente, corresponde a un análogo trifuncional de aminoácido y no a una impureza volátil o cromófora. Puede originarse en etapas de acoplamiento sintético o por desprotección incompleta en la fabricación de péptidos o aminoácidos. Sus múltiples centros ionizables confieren elevada polaridad, escasa retención cromatográfica y especiación dependiente del pH. La cuantificación suele requerir LC-MS/MS con cromatografía de interacción hidrofílica o LC con par iónico, junto con confirmación por MS de alta resolución y HPLC-UV tras derivatización.
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