La impureza Bacitracin EP Impurity B, también designada Bacitracin D2 o Bacitracin C3, es una variante peptídica estrechamente relacionada con bacitracina y registrada con el CAS 149146-35-0. Con fórmula molecular C64H99N17O16S y peso molecular de 1394.66 g/mol, pertenece al perfil de impurezas del antibiótico peptídico cíclico y puede originarse por microheterogeneidad biosintética, variantes del procesamiento enzimático o degradación menor durante la fermentación y el aislamiento. Su gran similitud estructural con bacitracina A dificulta la resolución cromatográfica y suele requerir LC de fase reversa de alta resolución con detección UV, LC-MS/MS y, para confirmación estructural, espectrometría de masas de alta resolución y análisis de fragmentación MS/MS. Su caracterización es relevante para el mapeo regulatorio de impurezas.
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