La impureza 3 de Milrinona (CAS 98293-81-3) | identificada como 5-cyano-6-oxo-1 | 6-dihydro-[3 | 4′-bipyridine]-2-carboxylic acid | es un subproducto polar de síntesis y degradación que requiere control analítico riguroso. Presenta grupos ciano y carboxilo que afectan su polaridad y acidez | condicionando su retención en cromatografía reversa y su ionización en espectrometría de masas. Se detecta y cuantifica mediante HPLC-UV y LC-MS con confirmación por RMN. Su presencia puede indicar incompleta ciclización u oxidación durante el proceso. El seguimiento conforme a ICH Q3A y límites de impurezas garantiza la calidad del principio activo.
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