N6-Metil-N6-nitroso-Adenosina (CAS 41286-73-1) es una impureza nitrosada crítica en perfiles de calidad de nucleósidos que exige control analítico riguroso. Esta nitrosamina derivada del N6 muestra alta polaridad y requiere técnicas sensibles como UHPLC-MS/MS o HPLC con detectores selectivos para cuantificación en trazas. Su formación suele originarse por nitrosación durante síntesis o purificación en presencia de donadores metilo y agentes nitrosantes. Por su potencial genotóxico debe ser evaluada conforme a guías regulatorias y sometida a estudios de estabilidad | límites especificados y procedimientos de remoción mediante optimización del proceso | lavado y agentes captadores. Monitorización y validación son esenciales.
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