Prucalopride Succinate Impurity - 2 corresponds to 4-Amino-5-chloro-2,3-dihydrobenzofuran-7-carboxylic acid, CAS 123654-26-2, with molecular formula C9H8ClNO3 and molecular weight 213.62 g/mol. This heteroaromatic aminobenzofuran carboxylic acid is consistent with a process-related intermediate or synthetic byproduct arising from incomplete functional group conversion during construction of the prucalopride scaffold. Its chlorinated amino acid-like framework warrants close monitoring because low-level aromatic amine impurities may require toxicological assessment under ICH M7 principles when present in active pharmaceutical ingredient streams. Characterization typically relies on LC-MS/MS, high-resolution LC-MS, and orthogonal HPLC-UV methods, with occasional confirmatory NMR for structural assignment.
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