Calcitriol EP Impurity A, 즉 trans-calcitriol은 이종 화학종의 분해물이 아니라 secosteroid 계열 비타민 D3 대사체에 속하는 입체이성질체 불순물이다. triene 시스템의 all-trans 배열은 calcitriol의 광화학적 또는 열적 이성질화에 의해 형성될 수 있으며, 합성, 저장, 또는 광노출 중에 상대적으로 증가할 수 있다. 분자식은 C27H44O3, 분자량은 416.6이며 CAS는 73837-24-8이다. 기하 이성질체는 UV 응답이 매우 유사하므로, 통상적인 규명 및 정량에는 키랄 또는 고해상도 역상 분리를 포함한 LC-MS/MS가 필요하며, NMR과 필요 시 유도체화 후 GC-MS가 보완적으로 사용된다.
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