Impureza (2S | 4R)-5-([1 | 1′-Biphenyl]-4-yl)-4-((tert-butoxycarbonyl)amino)-2-methylpentanoic Acid (CAS 1012341-54-6) é um subproduto estereoquímico com grupo Boc e núcleo bifenil que aumenta lipofilicidade e retenção em cromatografia reversa. Caracteriza-se por isomeria 2S | 4R e massa molecular compatível com detecção por LC-MS e HRMS | além de sinais diagnósticos em 1H e 13C NMR. Surge por epimerização parcial ou reação incompleta durante síntese de aminoácidos protegidos. Monitoramento por HPLC-UV | cromatografia quiral e análise por LC-MS é recomendado para controle de qualidade. Estratégias de controlo incluem purificação por cromatografia em fase sólida ou recristalização e estabelecimento de limites de impureza para garantia do perfil de pureza.
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![(2S,4R)-5-([1,1'-Biphenyl]-4-yl)-4-((tert-butoxycarbonyl)amino)-2-methylpentanoic Acid](https://veeprho.com/wp-content/uploads/2023/03/2S-4R-5-1-1-Biphenyl-4-yl-4-tert-butoxycarbonyl-amino-2-methylpentanoic-Acid-sqr.png "(2S,4R)-5-([1,1'-Biphenyl]-4-yl)-4-((tert-butoxycarbonyl)amino)-2-methylpentanoic Acid")
![(2R,4S)-ethyl 5-([1,1'-biphenyl]-4-yl)-4-amino-2-methylpentanoate](https://veeprho.com/wp-content/uploads/2023/11/VE0016600-2R-4S-ethyl-5-1-1-biphenyl-4-yl-4-amino-2-methylpentanoate.png "(2R,4S)-ethyl 5-([1,1'-biphenyl]-4-yl)-4-amino-2-methylpentanoate")
