Bacitracin EP Impurity K, também designada Bacitracin I3, é uma impureza peptídica derivada da bacitracina associada ao complexo antibiótico heterogéneo de dodecapeptídeos cíclicos. O CAS 144572-52-1 identifica este componente minoritário, com fórmula C64H94N16O17S e massa molecular de 1391,6 g/mol. A sua ocorrência é geralmente atribuída à heterogeneidade biossintética ou a deriva composicional pós-fermentativa, e não à degradação clássica de pequenas moléculas. Devido à sequência estreitamente relacionada e à complexidade isomérica entre congéneres de bacitracina, a caracterização requer normalmente LC-MS/MS com espectrometria de massa de alta resolução, complementada por RP-HPLC e, quando necessário, mapeamento peptídico. O enquadramento regulatório relaciona-se com o perfil de impurezas e a especificação de antibióticos polipeptídicos.
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