Alogliptin Impurity 35, CAS 872414-52-3, is a brominated heteroaromatic impurity with the molecular formula C8H8BrNO and molecular weight 214.1 g/mol. Its composition is consistent with a low-molecular-mass aryl bromide derived from synthetic intermediates or halogenation-related side reactions rather than a typical degradation product. The presence of bromine materially affects isotopic pattern assignment and chromatographic response. Characterization generally requires high-resolution LC-MS, LC-MS/MS, and orthogonal NMR confirmation, with impurity profiling by HPLC using UV and mass-selective detection. In a regulatory setting, such structurally defined impurities are assessed for process-related carryover and, where applicable, ICH M7 considerations.
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