Tri-beta-Alanin HCl, CAS 78089-27-7, ist das Hydrochloridsalz eines mehrbasischen Aminosäurederivats mit der Formel C9H17N3O4·HCl und einem Molekulargewicht von 231,25 + 36,46 g/mol. Strukturell handelt es sich um ein trifunktionelles Aminosäureanalogon und nicht um eine flüchtige oder chromophore Verunreinigung. Es kann aus Kupplungsschritten der Synthese oder aus unvollständiger Entschützung in der Peptid- oder Aminosäureherstellung entstehen. Die mehreren ionisierbaren Zentren bedingen hohe Polarität, geringe Retention und ausgeprägte pH-abhängige Speziation. Die Bestimmung erfolgt typischerweise mittels LC-MS/MS mit HILIC oder Ion-Pair-LC, ergänzt durch hochauflösende MS und derivatisierte HPLC-UV.
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