Bacitracin EP Impurity O, auch als Bacitracin J1 bezeichnet, ist ein höher homologes, zyklisches Dodecapeptid innerhalb des Bacitracin-A/B/C-Komplexes und entsteht typischerweise aus fermentationsbedingter Mikroheterogenität sowie postbiosynthetischer Prozessierung von Bacillus-licheniformis-Produkten. Mit der Summenformel C65H101N17O16S und einer Molmasse von 1408,7 g/mol besitzt es die charakteristische thiazolinhaltige Peptidarchitektur der Bacitracin-Familie. Die Strukturzuordnung und Spurenquantifizierung erfordern üblicherweise hochauflösende LC-MS, LC-MS/MS und Peptid-Mapping statt routinemäßiger UV-Methoden, da eng verwandte isobare Analoga und Sequenzvarianten vorliegen. Es handelt sich nicht um ein Nitrosamin und nicht um eine ICH-M7-DNA-reaktive Verunreinigung.
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