Bacitracin EP Impurity J, auch als Bacitracin I2 bezeichnet, ist eine eng verwandte zyklische Peptidverunreinigung des heterogenen Bacitracin-Komplexes. CAS 167289-63-6. Mit der Summenformel C64H94N16O17S und einer Molekülmasse von 1391,6 g/mol spiegelt sie feine Sequenz- und Oxidationszustandsunterschiede innerhalb der Decapeptid-Antibiotika wider und nicht einen einfachen niedermolekularen Abbauprodukt. Die Entstehung ist typischerweise mit biosynthetischer Diversität, fermentsbedingter Mikrohheterogenität oder geringfügigen Umwandlungen nach der Isolierung verknüpft. Für die Identifizierung sind RP-HPLC, LC-MS/MS, hochauflösende Massenspektrometrie und Peptidkartierung erforderlich, da UV-Nachweis isobare Bacitracin-Komponenten nicht ausreichend trennt.
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