6-Iodo Amiloride (CAS 60398-23-4) is a halogenated amiloride analog with the molecular formula C6H8IN7O and molecular weight 321.08 g/mol. As a positional iodinated derivative of the pyrazine-guanidinium diuretic scaffold, it is generally discussed as a synthetic-related impurity or process-related analog arising from electrophilic halogenation or incomplete substitution control during manufacture. Its polar, polybasic structure and heavy-atom iodine content can alter retention and ionization behavior relative to amiloride. Characterization and trace determination typically require LC-MS/MS, high-resolution mass spectrometry, and orthogonal LC-UV methods, with careful attention to isotopic patterns and fragment ions for assignment.
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