Bacitracin EP Impurity H는 Bacitracin H3로도 표기되며 CAS 144572-55-4에 해당하는 바시트라신 관련 리포펩타이드 변이체로, 폴리믹신 유사 펩타이드 항생제 불순물 프로파일에 포함된다. 분자식 C65H96N16O17S, 분자량 1405.6 g/mol이며, 비리보솜 펩타이드 생합성, 측쇄 절단, 또는 발효와 후속 정제 과정 중의 효소적 처리에서 기인하는 미세 이질성을 반영한다. 구조 규명에는 일반적으로 LC-MS/MS, 고분해능 질량분석, UV 검출을 갖는 역상 HPLC와 펩타이드 매핑이 요구된다. 미세한 서열 차이는 전하 상태, 크로마토그래피 거동, 활성 동등성에 영향을 줄 수 있어 약전 수준의 불순물 규명에서 중요하다.
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