Bacitracin EP Impurity J, designada también Bacitracin I2, es una impureza peptídica cíclica estrechamente relacionada con el complejo heterogéneo de bacitracina. CAS 167289-63-6. Con fórmula molecular C64H94N16O17S y peso molecular de 1391.6 g/mol, representa una variación sutil de secuencia y estado de oxidación dentro de la familia de antibióticos decapéptidos, más que un simple producto de degradación de baja masa. Su formación suele vincularse con divergencia biosintética, microheterogeneidad de fermentación o transformación menor tras el aislamiento. Su detección y cuantificación requieren RP-HPLC, LC-MS/MS, MS de alta resolución y mapeo peptídico, ya que la detección UV no resuelve componentes isobáricos de bacitracina.
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