Bacitracin EP Impurity J, also designated Bacitracin I2, is a closely related cyclic peptide impurity associated with the heterogeneous bacitracin complex. CAS 167289-63-6. With molecular formula C64H94N16O17S and molecular weight 1391.6 g/mol, it reflects subtle sequence and oxidation-state variation within the decapeptide antibiotic family rather than a simple low-mass degradant. Formation is typically linked to biosynthetic divergence, fermentation-related microheterogeneity, or minor post-isolation transformation. Characterization generally requires RP-HPLC, LC-MS/MS, high-resolution MS, and peptide mapping, because UV detection alone does not resolve isobaric bacitracin components.
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