Levofloxacin EP-Impurity E, auch Levofloxacin-Descarboxy-Impurity bzw. Decarboxy Levofloxacin genannt, ist das decarboxylierte Fluorchinolon-Analogon mit der CAS-Nummer 178964-53-9. Der Stoff bewahrt das Chinolon-Oxazin-Gerüst, besitzt jedoch nicht die Carboxylfunktion von Levofloxacin, entsprechend der Summenformel C17H20FN3O2 und einer Molekülmasse von 317,36 g/mol. Seine Entstehung wird meist mit synthetischen Nebenreaktionen sowie thermischer oder hydrolytischer Degradation während Herstellung und Lagerung in Verbindung gebracht. Die Identifizierung und Spurenbestimmung erfordern üblicherweise LC-MS/MS, HRMS und ergänzende orthogonale Verfahren, da konventionelle HPLC diese eng verwandte Spezies nicht immer eindeutig trennt.
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