El CAS 53-16-7 corresponde a la estrona, un esteroide estrogénico C18 de origen natural con fórmula molecular C18H22O2 y masa molecular de 270.37 g/mol. En el contexto farmacopeico se denomina Estriol EP Impurity B, Ethinylestradiol EP Impurity C y Estradiol EP Impurity A, ya que puede aparecer como impureza cetónica 17 por deshidrogenación oxidativa, control estereoquímico incompleto o interconversiones durante la síntesis y el almacenamiento de esteroides. Su cuantificación suele exigir HPLC o UPLC acoplada a LC-MS/MS, con confirmación estructural por HRMS y, cuando procede, RMN.
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