El N-óxido de levofloxacino, también denominado Impureza C EP de levofloxacino, es el derivado N-oxidado del núcleo de fluoroquinolona y corresponde a una degradación oxidativa del nitrógeno amínico terciario durante la fabricación o el almacenamiento. La sustancia, CAS 117678-38-3, presenta fórmula molecular C18H20FN3O5 y peso molecular 377.37 g/mol. Como impureza de oxidación y no como alerta estructural genotóxica, se evalúa en perfiles de impurezas y estudios de estabilidad. Su determinación suele requerir LC-MS/MS en fase reversa, LC-HRMS y HPLC ortogonal con detección por arreglo de diodos o fluorescencia, debido a su funcionalidad N-óxido polar y su comportamiento cromatográfico.
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