Sofosbuvir 5′-Desphosphate 3′-O-[(S)-Phosphate] | CAS 1337482-15-1 | es una impureza estructuralmente relacionada con el análogo nucleotídico Sofosbuvir que resulta de desfosforilación y refosforilación en la posición 3′. Esta impureza requiere evaluación rigurosa del perfil de impurezas por HPLC | LC-MS y NMR para determinar su presencia y niveles cuantitativos en materias primas y etapas intermedias. Sus propiedades fisicoquímicas y estabilidad frente a condiciones de proceso influyen en el desarrollo de métodos analíticos y criterios de aceptación. La identificación y cuantificación precisa son críticas para el control de calidad y la seguridad del producto final.
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