L-Lysine Diethylamide hydrochloride, CAS 266356-00-7, is a basic amide impurity of the lysine side chain in which both terminal amino hydrogens are replaced by ethyl groups, yielding the salt C10H25Cl2N3O with molecular weight 274.23 g/mol. Its occurrence may arise from stepwise N-ethylation during amide coupling or from residual alkylating reagents in synthesis. Although not a classical nitrosamine, its structural liability is relevant to impurity profiling because tertiary amide-related cationic species can complicate trace analysis. Definitive measurement generally requires LC-MS/MS, with orthogonal confirmation by high-resolution LC-MS or ion chromatography after salt characterization.
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