Fmoc-Dehydroxyserine (CAS 261522-33-2) | também conhecido como N-(9H-Fluorene-9-ylmethoxycarbonyl)-2 | 3-didehydro-L-alanine | é uma impureza de síntese originada pela eliminação do grupo hidroxila da serina protegida; esta forma insaturada exibe comportamento cromatográfico e espectrométrico diferenciado em relação ao Fmoc-Ser | sendo detectável por HPLC-UV e LC-MS através de padrões de fragmentação característicos do grupo Fmoc. Quimicamente mais reativa | pode sofrer adições de Michael e isomerizações | afetando a pureza e a integridade de lotes peptídicos se não controlada. Recomenda-se monitoramento por cromatografia líquida e otimização de condições de desproteção e armazenamento para minimizar sua formação.
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