Impureza 4-Nitrobenzyl (6R | 7R)-7-amino-3-cloro-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate Hydrochloride (CAS 53483-70-8) apresentada como subproduto de síntese pode afetar perfil de pureza e estabilidade do intermediário beta-lactâmico. Caracteriza-se por massa molecular e fragmentação específicas detectáveis por LC-MS e por tempo de retenção distinto em HPLC reverso com deteção UV a 254 nm. Sua formação está associada a reações incompletas de proteção/desproteção e nitratação do grupo benzílico. Estratégias de controle incluem otimização de condições de reação | purificação por cromatografia e especificação analítica com limites de aceitação. Avaliações de risco e estudos de degradação guiam mitigação e conformidade regulatória.
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![4-Nitrobenzyl (6R,7R)-7-amino-3-chloro-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate Hydrochloride](https://veeprho.com/wp-content/uploads/2023/12/4-Nitrobenzyl-6R-7R-7-amino-3-sqr.png "4-Nitrobenzyl (6R,7R)-7-amino-3-chloro-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate Hydrochloride")
