Sofosbuvir 3′ | 5′-Bis-(S)-phosphate (CAS 1337482-17-3) es una impureza fosforilada relacionada con la síntesis de sofosbuvir que presenta grupos fosfato en las posiciones 3′ y 5′ del nucleósido | conservando configuración S. Desde un punto de vista técnico representa un subproducto de sobrefosforilación o de reacción incompleta durante la etapa de formación del éster fosfato. Su identificación y cuantificación exigen técnicas analíticas sensibles como HPLC acoplada a MS | cromatografía de intercambio iónico y RMN para confirmar la estereoquímica. El control de proceso y la purificación mediante cromatografía específica son críticos para minimizar su presencia y asegurar la calidad del lote.
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![Sofosbuvir 5'-Desphosphate 3'-O-[(S)-Phosphate]](https://veeprho.com/wp-content/uploads/2022/07/Sofosbuvir-5-Desphosphate-3-O-5BS-Phosphate5D.png "Sofosbuvir 5'-Desphosphate 3'-O-[(S)-Phosphate]")
