Bacitracin EP Impurity G, also designated Bacitracin H2, CAS 144572-54-3, is a defined peptide-related impurity of the bacitracin polypeptide complex, consistent with an oxidation or hydrolytic variant rather than a simple process contaminant. With molecular formula C65H96N16O17S and molecular weight 1405.6 g/mol, it retains the cyclic lipopeptide architecture and sulfur-containing residue pattern characteristic of bacitracin congeners. Its assignment generally requires high-resolution LC-MS, LC-MS/MS, and orthogonal peptide mapping, because chromatographic coelution with closely related homologues is common. Regulatory characterization focuses on structural elucidation and impurity profiling rather than genotoxic concern.
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