Bacitracin EP Impurity K, also designated Bacitracin I3, is a bacitracin-derived peptide-related impurity associated with the heterogeneous cyclic dodecapeptide antibiotic complex. CAS 144572-52-1 identifies this minor component, with formula C64H94N16O17S and molecular weight 1391.6 g/mol. Its occurrence is generally attributed to biosynthetic heterogeneity or post-fermentation compositional drift rather than classical small-molecule degradation. Because of the closely related sequence and isomeric complexity within bacitracin congeners, characterization typically requires LC-MS/MS with high-resolution mass spectrometry, complemented by RP-HPLC and, when needed, peptide mapping. Regulatory assessment is relevant to impurity profiling and compositional specification of polypeptide antibiotics.
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