Bacitracin EP Impurity G, também denominada Bacitracin H2, CAS 144572-54-3, é uma impureza peptídica definida do complexo polipeptídico de bacitracina, compatível com uma variante oxidativa ou hidrolítica em vez de um contaminante de processo simples. Com fórmula molecular C65H96N16O17S e massa molecular de 1405.6 g/mol, preserva a arquitetura de lipopeptídeo cíclico e o padrão de resíduos contendo enxofre característicos dos congêneres de bacitracina. Sua atribuição geralmente requer LC-MS de alta resolução, LC-MS/MS e mapeamento peptídico ortogonal, pois a coeluição cromatográfica com homólogos muito próximos é frequente. O enquadramento regulatório concentra-se na elucidação estrutural e no perfil de impurezas, não em preocupação genotóxica.
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