Levofloxacin N-oxide, also designated Levofloxacin EP Impurity C, is the N-oxidized derivative of the fluoroquinolone scaffold, consistent with oxidative degradation of the tertiary amine nitrogen during manufacture or storage. The substance, CAS 117678-38-3, has molecular formula C18H20FN3O5 and molecular weight 377.37 g/mol. As an oxidation impurity rather than a structural genotoxic alert, it is generally evaluated within impurity profiling and stability studies. Determination typically requires reversed-phase LC-MS/MS, LC-HRMS, and orthogonal HPLC with diode-array or fluorescence detection, given its polar N-oxide functionality and chromatographic behavior.
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