Bacitracin EP Impurity K는 Bacitracin I3로도 표기되는 바시트라신 유래 펩타이드 관련 불순물로, 이질적인 환상 dodecapeptide 항생제 복합체의 구성 성분이다. CAS 144572-52-1, 분자식 C64H94N16O17S, 분자량 1391.6 g/mol로 정의된다. 그 존재는 전형적인 저분자 분해보다 생합성 이질성 또는 발효 후 조성 변동과 더 밀접하게 연관된다. 바시트라신 동족체 간 서열 유사성과 이성질체적 복잡성 때문에, 특성화와 정량에는 고분해능 질량분석을 결합한 LC-MS/MS가 통상 필요하며, RP-HPLC 및 필요 시 펩타이드 맵핑이 보조된다. 규제적으로는 폴리펩타이드 항생제의 불순물 프로파일과 조성 규격 설정에 중요하다.
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