La impureza EP K de bacitracina, también denominada Bacitracin I3, es una impureza peptídica derivada de bacitracina asociada al complejo antibiótico heterogéneo de dodecapéptidos cíclicos. El CAS 144572-52-1 identifica este componente menor, con fórmula C64H94N16O17S y peso molecular de 1391,6 g/mol. Su presencia se atribuye habitualmente a la heterogeneidad biosintética o a desviaciones composicionales posfermentativas, más que a una degradación clásica de moléculas pequeñas. Debido a la secuencia estrechamente relacionada y a la complejidad isomérica entre congenéricos de bacitracina, la caracterización suele requerir LC-MS/MS con espectrometría de masas de alta resolución, complementada por RP-HPLC y, cuando procede, mapeo peptídico. Su relevancia regulatoria reside en el perfil de impurezas y la especificación composicional de antibióticos polipeptídicos.
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