A Impureza EP E do acetato de hidrocortisona, também associada ao acetato de anecortave, é um éster acetato esteroidal relacionado ao arcabouço de corticosteroides e identificado pelo CAS 7753-60-8. Com fórmula molecular C23H30O5 e massa molecular de 386.48 g/mol, representa um perfil de impureza de glicocorticoide oxidado e acilado, e não um contaminante processual simples. Essas espécies podem resultar de desacetilação parcial, isomerização ou reações paralelas durante a síntese e o armazenamento. Pela semelhança estrutural com o esteroide de origem, a detecção em nível traço geralmente requer LC-MS/MS, LC-HRMS e métodos ortogonais de HPLC-UV para pureza de pico e caracterização de impurezas.
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