A Impureza G de Hidrocortisona Acetato EP, CAS 81968-66-3, é uma impureza esteroidal relacionada ao processo e à degradação da hidrocortisona acetato. Com fórmula molecular C25H34O7 e massa molecular de 446.53 g/mol, preserva o núcleo corticosteroide, mas apresenta padrões de acilação ou oxidação alterados, típicos de glicocorticoides semissintéticos. Essas impurezas podem formar-se durante a esterificação, o armazenamento ou sob estresse hidrolítico e oxidativo, e costumam exibir polaridade e comportamento cromatográfico muito semelhantes aos do fármaco principal. A elucidação estrutural e a quantificação exigem, em geral, HPLC em fase reversa ortogonal, LC-MS/MS e, quando necessário, RMN ou espectrometria de massas de alta resolução para distingui-la de análogos esteroidais relacionados.
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