Buspiron EP-Verunreinigung E, auch als Buspiron Open-Ring-Verunreinigung bezeichnet, ist eine strukturell definierte prozessbedingte Verunreinigung des azaspirodecandionhaltigen Anxiolytikums Buspiron, CAS 257877-43-3. Es handelt sich um ein offenkettiges Amid-Piperazin-Analogon, das durch Spaltung oder unvollständige Ringschlussbildung des pyrimidinyl-piperazinylischen Gerüsts während der Synthese oder unter hydrolytischen Abbau- und Lagerungsbedingungen entstehen kann. Die Verbindung besitzt die Formel C21H33N5O3 und eine Molekülmasse von 403,52 g/mol. Aufgrund der geringen Gehalte und der engen Strukturverwandtschaft zum Wirkstoff sind LC-MS/MS, hochauflösende MS sowie orthogonale RP-HPLC mit UV- oder CAD-Detektion für Identifizierung und Bestimmung erforderlich.
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