N-Nitrosopiperazine monomer, also known as mononitrosopiperazine, is a cyclic nitrosamine impurity with the molecular formula C4H9N3O and molecular weight 115.13 g/mol. CAS 5632-47-3 identifies this N-nitroso derivative of piperazine, a class associated with metabolic activation, DNA alkylation, and genotoxic concern under ICH M7 principles. It may arise from nitrosating conditions during synthesis or from contamination of piperazine-containing intermediates. Structural confirmation and trace quantification typically require LC-MS/MS or GC-MS, often with chromatographic separation optimized for volatile, low-level nitrosamines and careful control of artefactual nitrosation.
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