A Impureza A do Calcitriol EP, identificada como trans-calcitriol, é uma impureza estereoisomérica da família dos secosteroides metabólitos da vitamina D3, e não um produto de degradação de quimiotipos distintos. A geometria all-trans do sistema trieno pode resultar de isomerização fotoquímica ou térmica do calcitriol, com possível enriquecimento durante síntese, armazenamento ou exposição à luz. Apresenta fórmula molecular C27H44O3 e massa molecular 416.6, com CAS 73837-24-8. Como isómeros geométricos exibem respostas UV muito próximas, a caracterização geralmente exige LC-MS/MS com separação quiral ou de fase reversa de alta resolução, complementada por RMN e, quando necessário, GC-MS após derivatização.
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