Bacitracin EP Impurity E (CAS 22601-63-4) é uma impureza peptídica definida associada à bacitracina, com fórmula molecular C66H98N16O17S e massa molecular de 1419.67 g/mol. Trata-se de um análogo estruturalmente próximo e de maior massa do complexo peptídico cíclico da bacitracina, compatível com heterogeneidade relacionada ao produto gerada durante fermentação, processamento ou transformação proteolítica limitada. Devido à sua arquitetura peptídica polar e multifuncional, a caracterização normalmente requer LC em fase reversa acoplada a MS de alta resolução ou LC-MS/MS, com detecção UV ortogonal e, quando necessário, perfilamento por troca iônica. Em contexto monográfico, sua relevância está no acompanhamento de impurezas de processo e deriva composicional em materiais de bacitracina.
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