Mil = 5- methylene-L-isoleucine:4,10-Anhydro[N-[[(4R)-2-[(1S,2S)-1-amino-2-methylbutyl]-4,5-dihydro-1,3-thiazol-4-yl]carbonyl]-L-leucyl-D-α-glutamyl-L-isoleucyl-L-lysyl-D-ornithyl-5-methylene-L-isoleucyl-D-phenylalanyl-L-histidyl-D-α-aspartyl-L-asparagine]
Pharmacopeial Synonyms
Bacitracin J2
Sinônimos
N/A
Descrição
A Impureza EP P da bacitracina, também designada Bacitracin J2, é um peptídeo cíclico intimamente relacionado do complexo bacitracina, originado principalmente de microheterogeneidade biossintética e de variações da cadeia peptídica durante a fermentação ou a purificação subsequente. Com fórmula molecular C66H103N17O16S e massa molecular de 1422,70 g/mol, mantém a arquitetura peptídica sulfurada característica das bacitracinas, incluindo motivos tiazolínicos, distinguindo-se dos componentes majoritários por diferenças sutis de sequência e estado de oxidação. Sua elucidação e quantificação normalmente exigem RP-HPLC ortogonal, LC-MS/MS e espectrometria de massas de alta resolução, com mapeamento peptídico e fragmentação em tandem para confirmação estrutural e avaliação de abundância relativa.
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