Calcitriol EP Impurity B, también denominado 1β-Calcitriol o 1-epi-Calcitriol, es un metabolito estereoisomérico de la vitamina D3 con fórmula molecular C27H44O3 y peso molecular 416.64 g/mol, CAS 66791-71-7. Se diferencia del calcitriol por inversión en C1, situando el sustituyente hidroxilo en orientación β y modificando el reconocimiento del receptor y el comportamiento metabólico. Esta impureza suele asociarse con epimerización durante la síntesis o el almacenamiento de materiales secosteroideos. Dada la necesidad de separar epímeros muy próximos, su caracterización suele requerir LC-MS/MS, espectrometría de masas de alta resolución y HPLC quiral o en fase reversa con apoyo de RMN.
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